FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22082646
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG74400.047.5685594
CAAGTCG60000.024.9194
TAAGTCG24900.022.65060294
GAAGTCG107450.015.7468694
GTCCGAT14750.014.0203381
ATCGTAT18050.013.8005546
CGTATCG13400.013.6791043
AATCGTA25150.011.9602385
AAAGTCG37600.011.87594
CTCGGTT34900.011.4469921
GTCGGAA32550.011.2626721
GTTTATT96150.010.8029121
GTTCGAT25950.010.6859351
TCGTATC19900.010.628147
TTAGCGA18750.010.5280018
CTCGGAT59700.010.4706871
GTCGTTT19650.010.2849871
TCGGTTT33200.010.0512052
CGCGATT6554.9442133E-710.0458011
GGCGAAT68400.010.0321641
Produced by FastQC (version 0.11.5)