FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22555539
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG126250.056.21386394
CAAGTCG102200.029.43248694
TAAGTCG33750.028.2696394
GAAGTCG142900.022.2666294
AAAGTCG54850.016.023794
TACGCTA8200.014.902449
TATACGC8050.014.0124237
CGGAAAT40200.012.3930351
AATCGGG38700.012.3875965
GTCGAAT12650.012.260871
CGCGATT8250.011.9636361
CGAAGAT90100.011.9456171
GGAAAAT168550.011.6558891
GTTTTAT133650.011.2532731
GAAAAAT189000.011.2153441
TCGGATC44000.011.109092
CAATCGG28800.011.0972214
GTTTTTT256550.011.0469691
CGAAAAT30050.010.9484191
GTTTTAC65400.010.7798171
Produced by FastQC (version 0.11.5)