FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22835760
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG101700.051.71386394
CAAGTCG86800.029.8352594
TAAGTCG27900.025.77419394
GAAGTCG116450.017.11292594
AAAGTCG47350.014.095036594
CGCGTAT5059.0039975E-1013.9603961
GTTCGAT27700.013.4043321
AATCGTA19250.011.9636365
TTCGATT30000.011.9066672
CGGAAAT43150.011.3279261
GTCGGAT30000.011.1233331
TTAGCGA23050.010.8069418
TATACGG15300.010.75163434-35
GCGGTAT20650.010.6973361
GGCGAAT59000.010.5152541
ATCGTAT15400.010.3766226
TAGCGAG28250.010.3150439
CGCGTAA8752.8740033E-1010.2057131
GTCGGTT48200.010.0435681
TCGGATT25150.09.90457252
Produced by FastQC (version 0.11.5)