FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22213666
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG123450.040.77521594
CAAGTCG92900.023.72766594
TAAGTCG29100.021.96563594
GAAGTCG117800.017.03650394
AAAGTCG56450.013.23826594
GTCGGTT39100.012.6214831
TATACGC7401.6916601E-1011.4324337
CGCAAAT32550.011.4070671
CGAAAAT37200.011.3709691
GGAAAAT182250.011.1407411
GTTTAAT82350.011.1293261
CGGAAAT42850.011.078181
CGCGGAT27050.010.7726431
TTAGCGA14450.010.7335647
AAATCCA191450.010.605384
GTTTTAC66500.010.6015041
CTCAGAT168050.010.5438861
AATCCAA107750.010.5122985
CGAATCG14350.010.4808373
CTCGGAT61550.010.4614141
Produced by FastQC (version 0.11.5)