FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23731055
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG123700.052.62328794
CAAGTCG93200.028.08905894
TAAGTCG36800.024.77717694
GAAGTCG139450.019.34694
AAAGTCG51450.015.52964194
CGAAAAT40150.013.3449581
CGAATCG17600.013.0852273
GTCGGTT49550.012.7103941
GTTTTAT152400.012.70603751
GTCGAAC52000.012.5634621
TATACGC9750.012.5333357
CGCAAAT37600.012.51
GTTTATT104250.012.1726631
CGAAGAT92000.012.0565221
TACGCTA8250.011.9636369
AATCGTA21300.011.6948375
GTTTTTT303450.011.63191
CGCGATT7854.0017767E-1111.3757961
CGAAAAC37800.011.31481551
CTTTATT168800.011.0817541
Produced by FastQC (version 0.11.5)