FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23216438
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG86300.046.5098594
TAAGTCG29950.022.28380694
CAAGTCG68400.022.19444394
TACGCTA9000.017.7555569
ATATACG12500.016.926
GAAGTCG109150.016.57810494
GTTTTTT344000.016.5319771
TATACGC9750.016.3897447
CTCGATT51550.015.0436471
TTTTTTC470400.014.8573552
GTTTAAT110600.014.27848151
CGAAAAC37750.013.570861
TCGATTG24000.013.5125012
TTTTTTG504900.013.36742
CGAAAAT39400.013.3604051
AAATCCA216000.013.1208334
AATCCAC130350.013.0886085
CGCAAAT33150.013.043741
GAAAAAT242900.012.9641831
GCGAAAT30900.012.7766991
Produced by FastQC (version 0.11.5)