FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22515114
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG137550.060.61650594
CAAGTCG107400.029.53910494
TAAGTCG32600.026.52760594
GAAGTCG143500.024.59721494
AAAGTCG49700.019.2917594
GCGAAAT27250.016.385321
TTTTTTC367350.015.7754182
GTTTTTT287000.015.5902441
GCGGTAT24100.013.6514521
AAAAATC191750.013.579142
CGCAAAT30950.013.51534751
GTTTTAC56700.013.4285721
GAAAAAT200750.013.4151931
AAATCCA197300.012.8398374
TTTTTTG408200.012.4811372
GCGGAAT44600.012.4349781
CCCGAAC59700.012.3601341
GCCGAAT53200.012.1033831
GTTTAAT84500.012.0698221
CGAAAAC31700.012.0094631
Produced by FastQC (version 0.11.5)