FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22201766
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG94750.021.57783794
TACGCTA9150.019.5191259
TATACGC10350.016.8019317
CGCGAAT9850.016.223351
CTCGATT48600.014.5061731
GTTTTTT315400.014.2460371
CAAGTCG67150.013.92851794
AAATCGA29450.013.8845494
CGAAAAC36550.013.7592331
GCGAAAT24900.013.5903611
ATATACG13750.013.3309086
TAAGTCG26250.013.24952494
CGAAAAT44950.013.0700771
CTCAGAT170250.012.9750361
AAATCCA191000.012.8204184
GTTTTAT162550.012.5776681
CGCGTAT4506.1569153E-712.5333331
CTTAGAT70900.012.3963331
CGCAAAC38400.012.3619781
AAAAATC205050.012.0336492
Produced by FastQC (version 0.11.5)