FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22160300
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG102600.051.4892894
TAAGTCG31400.025.74522294
CAAGTCG77500.025.4103294
GAAGTCG119950.017.90662894
AAAGTCG48050.014.96566194
TACGCTA9200.013.2826099
TATACGC8950.013.1284927
GTCGGTT46100.013.0498911
ATATACG10950.012.4474896
CGAAAAT30700.012.2475571
GTTTTTT285850.011.7232821
GTCGAAT14050.011.7081861
CGAATCG17800.011.6179773
CGAAGAT87100.011.3857641
CGCGAAC14450.011.3840831
GTCGTAT12650.011.1462451
GTTTATT107150.011.0975271
GTTTTAC71550.010.9699521
GGAAAAT182400.010.8223691
TAAATCG12200.010.7868853
Produced by FastQC (version 0.11.5)