FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22160300
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG122400.039.85784594
CAAGTCG96750.023.3178394
TAAGTCG31100.021.91318394
GAAGTCG113750.015.45318794
GGCGAAT27800.012.6798561
CGAATCG14850.012.3434343
TATACGC8050.012.260877
CGAAAAT38500.011.84155751
CGGAAAC60100.011.2612311
CGCAATT20600.011.1796111
CGAAGAT80800.011.1683161
GTCGGTT38900.011.1156811
AAAGTCG52350.011.0429894
GGAAAAC154200.011.00324251
GTTTAAT79200.010.9785351
GTTTATT107250.010.9557111
CGGAAAT39750.010.8779871
CGAAAAC33700.010.7388731
GGAAAAT172700.010.668211
AATCCAA111000.010.4585585
Produced by FastQC (version 0.11.5)