FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22248876
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG110800.056.28971594
CAAGTCG84350.028.5287594
TAAGTCG34350.026.13391594
GAAGTCG128750.022.88854494
AAAGTCG50050.015.58841394
CGAAAAT34100.014.33431151
GTTTTTT299800.014.1721151
TTTTTTC376650.013.8011432
GCGAAAT30600.012.9019621
AAAAATC212850.012.7850612
GTCGATT19550.012.7416881
TTTTTTG414200.012.3003392
CGCATAT7701.8189894E-1212.2077931
AAATCCA190000.012.1705274
CGAAAAC30950.012.1486271
GAAAAAT218900.012.131111
AATCCAC107500.012.06697755
TTAGCGA24650.012.0121727
AAATCGA29850.011.8090474
CGCAAAT27300.011.7069611
Produced by FastQC (version 0.11.5)