FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22248876
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG135750.046.74033494
CAAGTCG102950.027.43759394
TAAGTCG35600.021.12359694
GAAGTCG128900.020.7106394
AAAGTCG56950.013.94732394
GTTTTTT277700.012.9812761
CGAAAAC31650.012.6224341
CGAAAAT45800.012.6222721
AAATCGA30150.012.3150914
CGGAAAT42850.012.2847151
GAAAAAT216350.011.8178881
AATCGGG41400.011.6932365
GCGAAAT31900.011.6394991
AAAAATC206800.011.6136372
CTCGATT38350.011.520211
GTTTTCT212450.011.238411
AAATCCA199200.011.1129524
AATCCAC117650.011.1058235
GTCGATT19150.011.0443881
GGAAAAT163900.011.0402681
Produced by FastQC (version 0.11.5)