FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22782277
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG119200.056.02936694
CAAGTCG93600.029.67628594
TAAGTCG34450.024.5573394
GAAGTCG130100.022.0730294
AAAGTCG51250.017.97463494
GTTTTTT309450.015.7957671
TTTTTTC414150.015.0481712
AAATCGA35850.014.6834044
CGCAAAT32450.014.1941451
AATCGAG36000.013.31666855
CGAAAAC32650.012.6676881
GTTTTAT166750.012.34542751
GGAAAAT189400.012.2338971
CGAAAAT37700.012.2175061
CGAATCG15900.012.1194983
GAAAAAT231800.012.0845571
GTCGAAT14400.012.0763891
TTTTTTG448800.012.0746442
GTTTAAT95800.012.0688941
GTTTTTC186100.011.996241
Produced by FastQC (version 0.11.5)