FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22782277
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG140000.047.6378694
CAAGTCG111900.027.42716894
TAAGTCG34750.025.29208894
GAAGTCG128200.020.09048594
CGAAAAC33600.016.086311
AAAGTCG58750.014.80000194
GTTTTTT310050.014.3857441
CGAATCG14500.012.9655173
AAATCGA32750.012.772524
GTTTTAC77550.012.3636361
CGCAAAC42300.012.2222221
GTTTTTC183100.011.9617711
CGGAAAT43250.011.9537571
GCGAAAT27400.011.8357671
GCCGATT23900.011.7991651
CTCGATT36700.011.3978211
CGAAAAT38450.011.36801051
GTTTAAT90200.011.3592021
GAAAAAT225550.011.2524951
CGAAGAT79450.011.2397741
Produced by FastQC (version 0.11.5)