FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23025672
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG96600.051.6708194
CAAGTCG74000.024.45270394
TAAGTCG31100.021.91318394
GAAGTCG110600.018.0605894
TTTTTTC592400.017.8035132
ATCAACG67500.017.6859265
TCAACGC66750.017.6734096
TTTTTTG775850.017.2407042
CAACGCA71350.017.1927137
TTTTTGA425200.017.1110084
AAAGTCG45700.016.86652294
TATACGC10050.016.8358217
TACGCTA10150.016.2068989
ACGCAAG73750.016.123399
TTTTTTA575050.015.7007232
TTTTGAG387800.015.3313575
CGAAAAT34750.015.1482011
ATTAAGC83400.015.0467636
AACGCAA78800.014.8515238
TAAGCAT74100.014.7786788
Produced by FastQC (version 0.11.5)