FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22393104
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG110650.052.9254494
CAAGTCG83650.026.29527994
TAAGTCG29900.023.10702194
GAAGTCG126400.018.55458894
GTTTTTT293150.015.872421
TTTTTTC385350.015.8679112
AAAGTCG49000.015.538774594
CGAAAAT36700.014.9836511
CGCGAAT5451.9099389E-1013.7981641
TTTTTTG417450.013.229132
TATACGC8450.012.7928997
AAAAATC203800.012.5225722
CGAATCG17000.012.1647063
GTTTTAC66150.011.9365071
CGCAAAT30000.011.9066671
CTTAGAT50200.011.8904381
GCGAAAT28800.011.751
GTTTAAT93050.011.71843051
GAAAAAT207850.011.5097431
CGCGAAC22600.011.4380541
Produced by FastQC (version 0.11.5)