FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23282120
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG79650.044.0200994
CAAGTCG59400.022.31313194
TATACGC10200.017.5098027
GTTTTTT316900.017.4562971
TACGCTA10800.016.5370379
GAAGTCG101550.016.47661294
TTTTTTC430100.016.2494772
CTCGATT49300.015.1582151
GTTTAAT102800.014.538911
TTTTTTG467900.014.5048092
AAAGTCG43700.014.41189994
GTTTTAC76700.013.9713161
CTTAGAT73100.013.5663481
CTCAGAT190600.013.3898211
CGAAAAT44950.013.383761
CTTAAAT93650.013.3497071
TTTTTTA447850.013.2336722
GTTTTTC169600.013.1910381
CGCAATC27350.013.06032851
GTTTTAT167050.012.8015571
Produced by FastQC (version 0.11.5)