FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23282120
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG107500.032.70325594
TAAGTCG29200.018.83219394
CAAGTCG80000.018.212594
TACGCTA10350.016.3478269
GTTTTTT343050.015.5776121
TATACGC11500.015.5304357
CGAAAAC40350.015.3754651
GAAGTCG101300.014.89338694
CGAAAAT46500.014.75698951
CTCAGAT188700.014.3216741
CGCAAAC41500.014.2698791
CTTAGAT69500.013.9985611
CTCGATT52100.013.8023021
GTTTTTC187150.013.460861
CGAAGAT91300.012.9726181
GCGAAAT27900.012.8028681
CGTGATA22700.012.4229089
CGAATCG16700.012.3832333
AAATCCA211500.012.3777784
CTTTGAT199500.012.3684211
Produced by FastQC (version 0.11.5)