FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21339525
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG171650.047.4791794
CAAGTCG121300.031.42374294
TAAGTCG37750.026.27019994
GAAGTCG147550.022.61606294
GCCGAAT55200.018.3913041
TATACGC12050.017.1618257
AAAGTCG65950.015.82107694
TACGCTA11200.015.5267869
CCGAATC70800.014.8036732
GCGAAAT28300.014.7809181
ATATACG13350.013.7303376
GTTTTTT255900.013.1871821
AAATCCA171350.012.8368844
CGAATCC51450.012.7891173
GGAAAAC130500.012.785441
CGAATCG15500.012.7354853
CGGACAT50850.012.6627331
AAAAATC164250.012.6191792
CTCGATT41150.012.563791
AATCCAC104200.012.5393485
Produced by FastQC (version 0.11.5)