FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23030567
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG101050.052.9302394
CAAGTCG88300.027.57191394
TAAGTCG32800.024.78963394
GAAGTCG107900.018.9916694
AAAGTCG51000.012.99411794
CGCGTAA8003.6379788E-1211.751
GTCGAAT16250.011.569231
GTCGTTT25150.011.3996031
AATCGTA25650.011.3606235
GTCGGTT42250.011.34674551
GTTCGAT27500.010.9381821
CGCGTTT13000.010.1230761
CGACCTA11800.09.9576274
GGCGAAT43700.09.8947361
CGCTTAA35050.09.78887244-45
CTCGTAT18400.09.7065221
CTCGGTT39300.09.6870221
GTCGGAA38350.09.6818771
GTCCGAT16100.09.633541
TCGGATT29400.09.5918362
Produced by FastQC (version 0.11.5)