FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23030567
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG123700.043.35246394
CAAGTCG99550.026.43897294
TAAGTCG34900.022.75931294
GAAGTCG121150.015.63433794
CGCGTAA5754.474714E-1013.078261
CGCGTAT4701.0418353E-612.01
AAAGTCG54900.011.471765594
CGCGGAT23900.011.2092041
AATCGTA21850.010.9702515
CTCGGTT38600.010.227981
CGGAAAT43900.010.0637811
CGGGGTT87100.010.0367391
GGCGAAT37800.09.8227521
CGGGAAT53300.09.7879921
GTCGTTT25650.09.71151
GCCGGTT47100.09.6794041
GGCGATT36950.09.5399191
TCGGATT28100.09.5338082
CGTATCG15900.09.459123
CGCGGGT52250.09.1751191
Produced by FastQC (version 0.11.5)