FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22080916
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG118450.055.63022294
CAAGTCG90300.028.2624694
TAAGTCG35550.022.60759594
GAAGTCG130300.021.82271894
AAAGTCG50250.016.4616994
GTCGGTT49650.013.8207451
CGCGTAT5101.5454134E-812.9019611
CGAAAAT32700.012.7920491
TACGCTA8850.012.7457629
ATATACG11850.012.6919846
GTCGAAT12650.011.8893281
GTTTATT100800.011.7966271
CGGAAAT42650.011.7913241
TATACGC8003.6379788E-1211.7500017
GTTTTTT275650.011.0999451
GCCGATT20900.011.0191391
CGAATCG18050.010.9362893
CTTTAAT108050.010.8745961
GGCGAAT45200.010.7101771
GGAAAAT175300.010.670851
Produced by FastQC (version 0.11.5)