FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22973096
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG120500.042.6315494
TAAGTCG30100.024.9833994
CAAGTCG88950.023.30185594
GAAGTCG121050.016.22965894
CGCGGAT24550.014.1670071
GTTCGAT34100.014.0586521
CGTATCG23400.013.4572663
GTCCGAT16400.013.1829271
CGCGTAT3701.4380617E-512.7027031
TTCGATT36700.012.1662132
AAAGTCG44950.012.02447294
TAGCGAG29750.012.0067229
TTAGCGA25450.011.4499028
TATACGG19450.011.23650434-35
CCGTATC33250.010.7428582
TCGGATT28550.010.5359032
TTAGACG24400.010.497951516-17
GTTTATT108450.010.3577691
AATCGTA18750.010.0266685
CGGAAAT43650.010.0137461
Produced by FastQC (version 0.11.5)