FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22974598
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG114750.056.60479494
CAAGTCG101550.031.24076894
TAAGTCG32250.024.77519294
GAAGTCG123350.020.1183694
AAAGTCG49250.013.93299594
GTTCGAT27950.012.77996351
GTCGGTT47800.012.1924691
GTCGAAT16200.011.89506151
CGCGTAT5202.7620263E-711.7499991
GGCGAAT45900.011.5708061
GTCCGAT15950.010.60815051
TCGGATT25500.010.5058812
GGGAAAT169700.010.4690631
TTCGATT30300.010.2376232
GTCGTAT12200.09.6311471
GTCGGAT27900.09.602151
GTCGGAG38000.09.5236841
GCCGGTT51650.09.4636981
CGTATCG14500.09.43
GGCGTTT56350.09.3416151
Produced by FastQC (version 0.11.5)