FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22391835
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG90250.043.43268694
CAAGTCG67850.022.58216794
TAAGTCG28150.016.0284294
GAAGTCG120650.015.9718294
CGAATCG15750.014.6222223
CGCGAAC12750.014.3764711
AAAGTCG43250.014.12716794
CGGAAAT45700.013.2669571
TATACGC8650.013.0404627
GTTTATT92200.012.9479391
CAATCGG33400.012.9461084
AATCGTA19550.012.7416885
AATCGGG48400.012.7210745
CTTTAAT86200.012.6496521
GTCGGTT47600.012.6386551
GTTTTAC58600.012.1109211
CGAAGAT93450.012.0706261
GTTTTTT252550.011.9477331
CGCAATT20550.011.8929431
CTCAGAT158900.011.5651361
Produced by FastQC (version 0.11.5)