FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22391835
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG116550.030.88974694
CAAGTCG85450.020.51609294
TAAGTCG27600.018.22101694
GAAGTCG112500.014.24622294
CGAATCG16950.013.3097353
GTTTAAT69450.012.3167751
CGGAAAT40950.012.0512831
GTTTATT94650.011.9672481
GGAAAAT153700.011.7117771
CTTTATT152250.011.6689641
CGCAAAT31000.011.5225811
CGAAGAT86800.011.4251151
CTCAGAT161150.011.37449551
TCGGATT27200.011.2316182
CGAAAAT41150.010.8505481
GGCGAAT43900.010.8132111
CTTTAAT86000.010.7662791
GTTTTTT246200.010.74776651
TATACGC9205.456968E-1210.7282617
GTCGGTT42400.010.641511
Produced by FastQC (version 0.11.5)