FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21281716
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG122850.059.52950794
CAAGTCG104550.031.6929794
TAAGTCG33300.023.5705794
GAAGTCG128400.020.35202494
AAAGTCG49850.017.1594894
GTCCGAT15200.012.9868431
CGCTAAT8350.012.9461081
GTTCGAT26350.012.1290321
CGTAACG4801.3431418E-611.753
GTCGGAT27900.011.4551971
GTCGAAT13200.011.3939391
CGCGTAA7201.2969394E-911.0972211
AATCGTA20300.010.8817735
GTCGGTT47250.010.8423281
GCGGTAT22350.010.5145421
TCGGATT25050.010.5069862
GTCGTTT16700.010.4131741
GCAAGTC115150.010.40816394
CGTATCG14800.010.1621633
GGGAAAT147050.010.0680051
Produced by FastQC (version 0.11.5)