FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21281716
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG140150.051.57759594
CAAGTCG115600.029.3953394
TAAGTCG34300.024.66472294
GAAGTCG121950.021.0045194
AAAGTCG51700.014.09090994
CGCGTAT4707.4040145E-813.0000011
TTAGCGA18450.012.2276428
CGCGTAA7853.6379788E-1211.9745231
GTCCGAT13500.011.8370371
GTCGGTT42800.011.5303741
GCGGTAT21300.011.4741791
GGCGATT38150.011.3342071
GTTCGAT26650.011.2870541
GGGAAAT133350.010.644171
CTCGGTT39650.010.549811
GGCGAAT42400.010.1981131
GCAAGTC115700.010.19619794
TAGCGAG23950.010.0083519
GCCGGTT54250.09.8764981
AATCGTA18300.09.7595635
Produced by FastQC (version 0.11.5)