FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22189184
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG111050.050.61863794
CAAGTCG83650.029.38553494
TAAGTCG32200.025.6894494
GAAGTCG131350.020.39588794
AAAGTCG49200.015.76219594
TACGCTA7400.014.6081089
TATACGC8800.013.8863647
GTCGGTT42100.012.8384791
CGAAAAT33300.012.1381381
CGAAGAT85000.011.8882341
CGCAATT23300.011.6995711
GTTTATT102050.011.3757951
GTTTAAT84700.011.3199531
GGCGAAT57450.011.2898171
CGCGGAT27000.011.1407391
AATCGGG41850.011.11827855
ATATACG11600.010.9396546
CGGAAAT38900.010.7532131
GTTTTTT294600.010.7369311
GGAAAAT167800.010.6996421
Produced by FastQC (version 0.11.5)