FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22189184
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG132950.040.79578894
CAAGTCG103050.027.00048394
TAAGTCG34850.024.41032894
GAAGTCG120300.019.06566894
TATACGC8900.014.2584267
CGAATCG16000.013.806253
CGCGAAT6651.8189894E-1213.428571
TACGCTA10300.013.2330099
CGAAAAT42350.012.8736721
AAAGTCG55150.012.61287394
CGGAAAT39400.012.525381
CGAAGAT84050.012.30221
ATATACG12850.012.0700396
GTCGGTT39200.011.6301021
GTTTATT94550.011.2342671
GGCGAAT46250.011.1783771
CTTTATT145600.010.5879121
CTCAGAT171150.010.4902131
GTTTTAC63700.010.4034531
TCGGATT26750.010.3663552
Produced by FastQC (version 0.11.5)