FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21630194
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG172450.059.49608694
CAAGTCG115800.031.00863394
TAAGTCG47950.030.0917694
GAAGTCG175000.025.99771394
AAAGTCG57300.019.68586394
GTATACG14850.012.0269367
TTTTCGA43950.011.9772477
CGCGTTA4851.5218138E-611.6288657
ATTTTCG47450.011.2918856
TATACGG16100.011.0931688
TACGATT16600.010.475903594
TAAGACG15450.010.0388358
ACAAGTC128950.010.02326494
ATAAGTC54600.09.64102592-93
AGAAGTC248300.09.54007294
TTTCGAT57450.08.9991298
TATATCG7957.188464E-78.8679257
CGAAGTC91000.08.85769292-93
TTAAGTC68700.08.75691492-93
CGCGTAA7050.08.66666688-89
Produced by FastQC (version 0.11.5)