FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21630194
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT14750.018.4813561
GTTTTTT275200.015.1144621
CGCAAAC43650.014.3207331
AAATCGA32600.014.2730064
CTCGATT34800.013.10057451
CGAAAAC34800.012.9655171
CGCAAAT30100.012.8039871
CTTTAAC70550.012.7243081
CGAAAAT44600.012.645741
GCGAAAT26750.012.4747661
GTTTAAT81150.012.4522481
CTTAGAT49500.012.1535351
CTCAGAT168850.012.0805441
CGAATTG27500.011.9636353
CGAAGAT97000.011.9195861
AATCGAG35250.011.7333345
GGAAAAC148400.011.5283011
CTCGGAT63550.011.3154991
GGAAATT106600.011.2870541
AAATCCA182500.011.284
Produced by FastQC (version 0.11.5)