FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22853920
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG99550.050.80060294
CAAGTCG83100.028.78820894
TAAGTCG28700.023.09059194
GAAGTCG114700.018.68526694
TTAGCGA23500.013.28
GTTCGAT25700.012.6186781
GTCGGTT48000.011.7499991
AAAGTCG46950.011.7124694
TAGCGAG28550.011.6882669
TCGCGTA6704.982212E-911.2238812
CGCGTAT5556.4157757E-711.0090091
TCGCATA7854.7293724E-1010.777072
GTCGGAT27900.010.6129041
GGCGGAT91150.010.5704881
TTCGATT29450.010.5331062
GTCGAAT12550.010.4860561
GGCGAAT54300.010.4732961
AATCGTA21600.010.4444455
CGGAAAT44550.010.3389461
GCGGTAT20150.010.2630281
Produced by FastQC (version 0.11.5)