FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22210190
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG174850.045.8575994
CAAGTCG121800.029.98275894
GAAGTCG146100.022.39014494
TAAGTCG38300.021.96605594
GCGAAAT33350.019.8710631
AAAGTCG68600.016.30612294
GCCGAAT66800.015.54941
GTTTTTT285350.014.8897841
GCGGAAT53700.014.5288641
CTCGATT45750.014.1770491
CGGATAT26150.013.4799231
GCGGTAT38450.013.44603351
AAATCCA185700.013.1610124
CGCGAAC14300.013.14685251
CGAAAAC41700.013.0743411
CGAAATA26700.013.02621652
AATCCAC113650.012.9441275
CGAAAAT44450.012.8998871
CGGTATA12400.012.8870962
AAAAATC195350.012.6552332
Produced by FastQC (version 0.11.5)