FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23471957
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG128850.055.00659694
CAAGTCG98950.027.54926794
TAAGTCG35350.026.1923694
GAAGTCG144600.022.42738594
AAAGTCG55750.015.93363194
CGAATCG16450.015.4285713
CGCAAAT31150.013.881221
AATCGGG48900.013.840495
TATACGC8450.012.7928997
CGAAAAT37950.012.7562571
CGCGATT9450.012.4338621
CGGAAAT46750.011.7625671
TCGAATC20150.011.662532
TACGCTA8157.2759576E-1211.5337429
CGCGTAT6603.936293E-911.3939391
GTTTATT103500.011.3072451
GTTTTTT290200.011.1912461
AATCGTA19400.011.1443295
CGAAGAT91150.011.1376851
GTCGTAT11550.010.9870131
Produced by FastQC (version 0.11.5)