FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23471957
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG144050.048.32141594
CAAGTCG115900.027.77825794
TAAGTCG35650.024.12622694
GAAGTCG132350.019.49603394
AAAGTCG58100.014.72289294
CGCGTAT5302.6446287E-812.4150931
CGGAAAT45550.011.866081
CGAAGAT86600.011.8314091
GTCGGTT46300.011.6738661
CGAATCG15500.011.522583
TCGGATT26250.011.4590462
CTCGGAT64350.011.1748261
CGCAAAT33300.011.0090081
CGCGTAA8551.6370905E-1110.9941511
GTTTTAT140100.010.9029261
GGAAAAT178250.010.8633941
GTTTTTT282150.010.7942581
AAATCGA32400.010.7345684
CGCTAAT9701.8189894E-1210.6597931
TTAGCGA19500.010.6051267
Produced by FastQC (version 0.11.5)