FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21595266
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG106350.057.540294
CAAGTCG84150.027.6470694
TAAGTCG32600.025.23006294
GAAGTCG116500.024.28669794
GTTTTTT310950.015.62888051
TTTTTTC431400.015.1764032
TACGCTA8100.015.0864229
CGAAAAT32550.014.4393241
CGCGAAT7250.014.262071
AAAGTCG46950.013.71459194
TTTTTTA434550.013.400762
TTTTTTG483050.013.3785322
GCGAAAT25800.013.116281
GTTTAAT92100.012.4006531
AAATCGA29650.012.364254
GAAAAAT210200.012.2083731
GTCGATT19650.011.9592881
AAATCCA194300.011.9495634
CGCAAAC38000.011.8736851
GGAAAAT163650.011.8612911
Produced by FastQC (version 0.11.5)