FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21595266
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG140150.044.2668694
CAAGTCG105250.023.2655694
TAAGTCG36950.022.64140794
GAAGTCG124200.020.62399594
AAAGTCG54500.015.52293794
GTTTTTT284400.013.6339661
CTCGATT37750.013.3218551
CGCGAAT7450.013.2483231
CGAAAAT40500.012.7654331
CGGAAAT41500.012.6843391
CGCAAAC41700.012.3980821
CTCGGAT65300.012.0918841
CGAAAAC34750.011.9021591
AAATCCA201350.011.6945624
AAATCGA31350.011.6937814
CGGATAT21750.011.6689661
CTTAGAT59950.011.6030031
CTCAGAT159250.011.5987441
GTTTAAT87850.011.5025621
GCGAAAT26550.011.3295681
Produced by FastQC (version 0.11.5)