FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23519789
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG143800.055.89012594
CAAGTCG116650.030.25889294
TAAGTCG39600.028.95959594
GAAGTCG160000.025.82062594
AAAGTCG59600.018.68959694
GTTTTTT263800.014.0750561
TTTTTTC363650.013.69998552
CGCGAAT14450.013.6608991
CGCGATT8650.013.5838141
CGCGAAC13600.013.1323521
GTTTAAT74900.013.114821
CGCGTAT4002.2553795E-612.9251
TTTTTTG389150.012.6694062
GCGAAAT28750.012.4243471
GTCGAAC48850.011.7379721
CGAAAAC37450.011.6715621
AAAAATC194100.011.5744462
CGCAAAC39900.011.54385951
GGAAAAT178550.011.4768961
GAAAAAT201650.011.4673941
Produced by FastQC (version 0.11.5)