FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22066638
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG72150.037.2612694
CAAGTCG54700.019.93418794
TATACGC8750.016.6514287
CTCGATT47100.016.1656061
TACGCTA8300.015.8554239
GTTTTTT321550.015.8298861
TTTTTTC430400.015.2225832
ATATACG12100.014.760336
TAAGTCG23000.014.50869794
GTTTAAT100400.014.4651391
GAAGTCG89800.014.39309594
TCGATTG24900.014.3453812
CGTATCG16850.014.22551950-51
TTTTTTG461450.013.9538422
CGAAAAC34600.013.4479771
CTTAGAT63350.013.2059981
TTTTTTA449850.013.1643892
CGCAATT20350.012.93366051
GTTTTAT176850.012.8894551
AAATCGA33550.012.8882274
Produced by FastQC (version 0.11.5)