FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22066638
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG95850.028.0479994
TACGCTA9300.020.7204329
TATACGC10750.018.87
TAAGTCG23100.017.29437394
CTCGATT47700.016.1593281
ATATACG13450.015.7249076
GTTTTTT331200.015.4396131
CAAGTCG71350.014.49194194
CGCAAAC37150.014.0430691
CGAAAAT44950.013.8020031
CGAATCG16650.013.5495493
GAAGTCG94300.013.50689394
CGAAAAC33900.013.4483781
CTTAGAT72750.013.1147771
GGAAAAT161450.012.92536451
CGCAAAT35000.012.7571431
AAATCCA191450.012.4465914
CGCGAAT14900.012.3020131
GTTTAAT90900.011.99561
CTCAGAT165050.011.9315371
Produced by FastQC (version 0.11.5)