FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22456922
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG150900.048.6507694
CAAGTCG112750.027.05365894
GAAGTCG135450.022.5544594
TAAGTCG39100.019.8337694
CGCAAAC42250.015.351481
GTTTTTT320600.014.4694321
AAAGTCG66100.014.22087794
CGGATAT25850.014.1818181
CGAAGAT84100.013.6920331
CGCGTTT16050.013.177571
GCGAAAT30300.013.0297031
CGAATCG17600.012.0170453
CGGAAAT49400.011.8927131
CGAAAAT44400.011.8558561
GTTTAAT99300.011.7854991
CTTTAAC85850.011.7157821
CGAAAAC37450.011.6715621
AAATCGA36600.011.5573784
AAATCCA209900.011.5540734
CTCAGAT175800.011.4425491
Produced by FastQC (version 0.11.5)