FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23121496
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG98650.047.3096894
CAAGTCG80300.025.28518194
GAAGTCG111450.020.8326694
TAAGTCG32500.017.35384894
GTTTTTT322000.016.4645981
GCGAAAT31500.014.9206351
TTTTTTC429800.014.7954862
CTTAGAT74900.014.4953271
CGAAAAT44450.014.3802021
TTTTTTG462500.013.9526492
TATACGC10500.013.8761917
CGCAATC30150.013.562191
GTTTAAT108350.013.4471631
CTCAGAT175900.013.2797051
CTCGATT51050.013.2575911
CGCAAAT35500.013.1070421
GAAAAAT262500.013.1062861
GTTTTAT180200.013.0671481
AAAGTCG49100.013.01833194
GTTTTAC76250.013.0059021
Produced by FastQC (version 0.11.5)