FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23121496
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG130700.035.5646594
CAAGTCG97400.022.05236294
TAAGTCG36150.020.2821694
GAAGTCG111100.017.85238694
CGCGAAT4401.1277734E-1016.0227281
CGAATCG16100.015.472053
GTTTTTT338900.015.3800541
TACGCTA12300.015.2845539
GCGAAAT25800.014.3914731
CGAAAAC39300.014.1119591
TATACGC13050.014.0459787
CTCGATT50300.014.0159041
CGAAAAT42950.013.2409771
ATATACG14300.013.1468546
CGCGAAC15800.012.791141
CTCAGAT180900.012.7307921
AAATCGA35550.012.42756754
CTTAGAT76500.012.2875811
GTTTAAT101550.012.2186121
AAAGTCG54850.011.910665594
Produced by FastQC (version 0.11.5)