FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21713546
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG101350.045.6319794
TAAGTCG27450.023.28597594
CAAGTCG85550.022.5248494
GAAGTCG111200.018.21672694
GTTTTTT262400.016.4070111
TATACGC9800.015.3469397
TACGCTA10750.014.8651169
GCGAAAT23550.014.5690021
CTTAGAT68950.014.4510521
TTTTTTC353600.014.2621612
AAATCGA29200.014.1643834
AAAGTCG42050.013.97146394
CTCGATT47200.013.5423741
AAATCCA178050.013.5417024
AATCGGG37000.013.4648665
CGAAAAC37500.013.41066551
ATATACG12750.013.2705886
GTTTAAT85700.013.107351
TTTTTTG382700.012.9811872
GAAAAAT197750.012.5254121
Produced by FastQC (version 0.11.5)