FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21713546
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG130350.035.44380694
TAAGTCG28650.021.16230494
CAAGTCG99150.020.80988394
TACGCTA10450.017.5406689
TATACGC11100.017.360367
GAAGTCG113850.016.71936894
GTTTTTT279350.015.445141
CTCGATT46350.015.3117591
AAATCGA31550.014.7480184
ATCGAGT35200.013.8863646
CTCGAAT33700.013.3887241
GTTTTAT140250.013.2370771
AATCGAG36550.012.9876875
TCGAATC16650.012.9849852
AAATCCA174150.012.9813384
AATCGGG34050.012.8370065
GCGAAAT26000.012.8346161
AATCCAA91250.012.7736985
CGAATTG21050.012.5035633
AAAAATC176450.012.3859452
Produced by FastQC (version 0.11.5)