FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22664542
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG154200.045.04928294
CAAGTCG107000.026.57476694
TAAGTCG44800.020.87723294
GAAGTCG133900.020.4637894
AAAGTCG63500.014.58110294
CGCGATT7850.013.77071
TATACGC10900.012.935787
TCGGATT28850.012.3812822
CGAAGAT81050.012.2936461
ATATACG13800.011.9202896
CGAAAAT38000.011.7499991
CGGAAAT41750.011.7077851
GGCGAAT36150.011.5712311
CTCGGAT69150.011.4186541
GCGGTAT25850.011.2727271
CGCGGAT25900.011.2509651
GGAAAAT204650.010.9318351
CGAAAAC37850.010.9273451
AAATCGA35700.010.9271714
GGAAAAC158850.010.6219711
Produced by FastQC (version 0.11.5)