FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22140368
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG151600.063.7104294
CAAGTCG114450.031.70292794
TAAGTCG41600.027.11538194
GAAGTCG154600.025.87127994
AAAGTCG57200.021.61013894
GTTTTTT267500.016.4807471
GCGAAAT29450.016.438031
TTTTTTC353650.015.4429522
CGCGAAT13350.014.7865161
GCCGAAT52750.013.9886251
AAAAATC185050.013.9183992
GAAAAAT187350.013.8980521
AAATCCA175000.013.6434294
GTCGATT20300.012.733991
GTTTAAT77850.012.5574831
GGAAAAT154050.012.53943351
TTTTTTG388050.012.3298542
CGAAAAT34800.012.290231
AATCCAC102650.012.2250375
GTTTTCT206100.012.2231921
Produced by FastQC (version 0.11.5)