FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22140368
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG179550.053.76663694
CAAGTCG138850.028.77205394
TAAGTCG41250.026.54787894
GAAGTCG152400.024.39435694
AAAGTCG62800.017.58757894
CGCGAAT12000.016.8416671
GCGAAAT26050.014.4337811
GTTTTTT262500.013.82247451
CGAAAAT46100.013.2537951
CGCGAAC14000.013.0928561
AAATCCA180150.012.5750754
GCCGAAT50400.012.4960311
AAATCGA30200.012.4503314
CGGAAAT41950.012.3241951
CGCAAAC43200.012.1851851
GAAAAAT182800.012.1613781
GTTTTCT192150.012.1321881
AATCCAC109150.011.9276235
GGAAAAT151050.011.7616681
AAAAATC177150.011.70025352
Produced by FastQC (version 0.11.5)