FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22955473
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG119550.054.8824894
CAAGTCG95300.026.28646794
TAAGTCG35950.023.40194994
GAAGTCG137650.021.10134594
TATACGC8950.016.279337
AAAGTCG48900.016.24335594
CGCGAAT12800.013.9531251
TACGCTA7900.013.6835459
GTTTTTT289000.012.7176481
ATATACG12450.012.4578326
CGCAAAT33850.012.2186121
CGAAAAT31600.011.8987351
GTTTATT98500.011.8812181
GTCGGTT46350.011.8640781
CGGAAAT40600.011.8078831
AATCGGG39050.011.6747765
CGAAAAC33850.011.3855251
AATCGTA19900.011.3366835
CGAAGAT88050.011.1561621
GTCGGGT51600.010.83914851
Produced by FastQC (version 0.11.5)